Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach
Identifieur interne : 004824 ( Main/Exploration ); précédent : 004823; suivant : 004825Generation of human antibody fragments recognizing distinct epitopes of the nucleocapsid (N) SARS-CoV protein using a phage display approach
Auteurs : Michela Flego [Italie] ; Paola Di Bonito [Italie] ; Alessandro Ascione [Italie] ; Silvia Zamboni [Italie] ; Alessandra Carattoli [Italie] ; Felicia Grasso [Italie] ; Antonio Cassone [Italie] ; Maurizio Cianfriglia [Italie]Source :
- BMC Infectious Diseases [ 1471-2334 ] ; 2005.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (immunologie), Banque de peptides, Fragments d'immunoglobuline (immunologie), Humains, Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie), Épitopes (génétique), Épitopes (immunologie).
- MESH :
- génétique : Protéines nucléocapside, Virus du SRAS, Épitopes.
- immunologie : Anticorps antiviraux, Fragments d'immunoglobuline, Protéines nucléocapside, Virus du SRAS, Épitopes.
- Banque de peptides, Humains, Protéines nucléocapside.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Epitopes, Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Viral, Epitopes, Immunoglobulin Fragments, Nucleocapsid Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus.
- Humans, Peptide Library.
Abstract
Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. Successful control of the global SARS epidemic will require rapid and sensitive diagnostic tests to monitor its spread, as well as, the development of vaccines and new antiviral compounds including neutralizing antibodies that effectively prevent or treat this disease.
The human synthetic single-chain fragment variable (scFv) ETH-2 phage antibody library was used for the isolation of scFvs against the nucleocapsid (N) protein of SARS-CoV using a bio panning-based strategy. The selected scFvs were characterized under genetics-molecular aspects and for SARS-CoV N protein detection in ELISA, western blotting and immunocytochemistry.
Human scFv antibodies to N protein of SARS-CoV can be easily isolated by selecting the ETH-2 phage library on immunotubes coated with antigen. These
The human scFv antibodies isolated and described in this study represent useful reagents for rapid detection of N SARS-CoV protein and SARS virus particles in infected target cells.
The online version of this article (doi:10.1186/1471-2334-5-73) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2334-5-73
PubMed: 16171519
PubMed Central: 1261265
Affiliations:
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Le document en format XML
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(genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Peptide Library</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (immunologie)</term><term>Banque de peptides</term><term>Fragments d'immunoglobuline (immunologie)</term><term>Humains</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Épitopes (génétique)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Epitopes</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Epitopes</term><term>Immunoglobulin Fragments</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments d'immunoglobuline</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Peptide Library</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Humains</term><term>Protéines nucléocapside</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Severe acute respiratory syndrome (SARS)-CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. Successful control of the global SARS epidemic will require rapid and sensitive diagnostic tests to monitor its spread, as well as, the development of vaccines and new antiviral compounds including neutralizing antibodies that effectively prevent or treat this disease.</p></sec><sec><title>Methods</title><p>The human synthetic single-chain fragment variable (scFv) ETH-2 phage antibody library was used for the isolation of scFvs against the nucleocapsid (N) protein of SARS-CoV using a bio panning-based strategy. The selected scFvs were characterized under genetics-molecular aspects and for SARS-CoV N protein detection in ELISA, western blotting and immunocytochemistry.</p></sec><sec><title>Results</title><p>Human scFv antibodies to N protein of SARS-CoV can be easily isolated by selecting the ETH-2 phage library on immunotubes coated with antigen. These <italic>in vitro</italic> selected human scFvs specifically recognize in ELISA and western blotting studies distinct epitopes in N protein domains and detect in immunohistochemistry investigations SARS-CoV particles in infected Vero cells.</p></sec><sec><title>Conclusion</title><p>The human scFv antibodies isolated and described in this study represent useful reagents for rapid detection of N SARS-CoV protein and SARS virus particles in infected target cells.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2334-5-73) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name></author><author><name sortKey="Heinen, P" uniqKey="Heinen P">P Heinen</name></author><author><name sortKey="Iturriza Gomara, M" 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Cao</name></author><author><name sortKey="Wong, Ty" uniqKey="Wong T">TY Wong</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Xie, Y" uniqKey="Xie Y">Y Xie</name></author><author><name sortKey="Guo, Z" uniqKey="Guo Z">Z Guo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Flego, Michela" sort="Flego, Michela" uniqKey="Flego M" first="Michela" last="Flego">Michela Flego</name></noRegion><name sortKey="Ascione, Alessandro" sort="Ascione, Alessandro" uniqKey="Ascione A" first="Alessandro" last="Ascione">Alessandro Ascione</name><name sortKey="Carattoli, Alessandra" sort="Carattoli, Alessandra" uniqKey="Carattoli A" first="Alessandra" last="Carattoli">Alessandra Carattoli</name><name sortKey="Cassone, Antonio" sort="Cassone, Antonio" uniqKey="Cassone A" first="Antonio" last="Cassone">Antonio Cassone</name><name sortKey="Cianfriglia, Maurizio" sort="Cianfriglia, Maurizio" uniqKey="Cianfriglia M" first="Maurizio" last="Cianfriglia">Maurizio Cianfriglia</name><name sortKey="Di Bonito, Paola" sort="Di Bonito, Paola" uniqKey="Di Bonito P" first="Paola" last="Di Bonito">Paola Di Bonito</name><name sortKey="Grasso, Felicia" sort="Grasso, Felicia" uniqKey="Grasso F" first="Felicia" last="Grasso">Felicia Grasso</name><name sortKey="Zamboni, Silvia" sort="Zamboni, Silvia" uniqKey="Zamboni S" first="Silvia" last="Zamboni">Silvia Zamboni</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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